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位置: 首页 > 重组兔单抗 > Wilms Tumor Protein Rabbit mAb

Wilms Tumor Protein Rabbit mAb#48959

评价
Recombinant
Wilms Tumor Protein Rabbit mAb
Wilms Tumor Protein Rabbit mAb
Wilms Tumor Protein Rabbit mAb
Wilms Tumor Protein Rabbit mAb
Wilms Tumor Protein Rabbit mAb
Wilms Tumor Protein Rabbit mAb
Wilms Tumor Protein Rabbit mAb
Wilms Tumor Protein Rabbit mAb
是否有货: Yes
产品总价:
产品详情

产品名称Wilms Tumor Protein Rabbit mAb

克隆号SC06-41

纯化Affinity-chromatography

应用WB, ICC/IF, IHC, FC

种属反应性Hu, Ms

免疫原描述A synthesized peptide derived from human WT1

别名WIT 2 antibody
WT 1 antibody
AWT1 antibody
FWT1 antibody
GUD antibody
NPHS4 antibody
WAGR antibody
Wilms tumor 1 antibody
Wilms Tumor antibody
Wilms tumor protein antibody
Wilms' tumor gene antibody
Wilms' tumor protein antibody
WIT2 antibody
WT antibody
WT1 antibody
WT1_HUMAN antibody
WT33 antibody

数据库入口号Swiss-Prot#:P19544

计算分子量55 kDa

配方Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

应用详情

WB 1:1000-1:2000
IHC 1:100-1:200
ICC/IF 1:50-1:200
FC 1:20-1:100

ICC staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Western blot analysis of WT1 expression in K562 cell lysate.
Immunohistochemical analysis of paraffin-embedded mouse kidney, using Wilms Tumor Protein Antibody.
背景

Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation.

背景文献

1. Witty AD et al. Generation of the epicardial lineage from human pluripotent stem cells. Nat Biotechnol 32:1026-35 (2014).
2. Gao Y et al. WT1 regulates the development of the posterior taste field. Development 141:2271-8 (2014).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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