产品详情
产品名称DIABLO Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号SC67-06
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名0610041G12Rik antibody
DBLOH_HUMAN antibody
DBOH antibody
DFNA64 antibody
diablo antibody
Diablo homolog (Drosophila) antibody
Diablo homolog antibody
Diablo homolog mitochondrial antibody
Diablo IAP binding mitochondrial protein antibody
Diablo like protein antibody
DIABLO S antibody
Direct IAP binding protein with low pI antibody
Direct IAP-binding protein with low pI antibody
FLJ10537 antibody
FLJ25049 antibody
mitochondrial antibody
Mitochondrial Smac protein antibody
Second mitochondria derived activator of caspase antibody
Second mitochondria-derived activator of caspase antibody
SMAC 3 antibody
Smac antibody
Smac protein antibody
SMAC3 antibody
数据库入口号Swiss-Prot#:Q9NR28
计算分子量21 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of DIABLO on different lysates using anti-DIABLO antibody at 1/1,000 dilution. Positive control:
Lane 1: Jurkat
Lane 2: Hela
Lane 3: MCF-7
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse overy tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
ICC staining DIABLO in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining DIABLO in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining DIABLO in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining DIABLO in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of HepG2 cells with DIABLO antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
The activation of caspases is a key regulatory step in apoptosis. Once cytochrome c is released from the mitochondria into the cytosol, it binds Apaf-1 to form an oligomeric cytochrome c/Apaf-1 complex, which induces caspase activation. Inhibitors of Apoptosis Proteins (IAPs), are a family of proteins that regulate the cytochrome c/Apaf-1 caspase activating pathway. Like cytochrome c, Smac (for second mitochondria-derived activator of caspase, also designated DIABLO in mouse for direct IAP binding protein with low PI) promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding IAPs and preventing them from inhibiting caspases. In healthy cells, Smac is a mitochondrial protein, but when cells undergo apoptosis, Smac is released into the cytosol.
背景文献
1. Masoumi KC et al. Identification of a novel protein kinase Cd-Smac complex that dissociates during paclitaxel-induced cell death. FEBS Lett 586:1166-72 (2012).
2. Lamers F et al. Identification of BIRC6 as a novel intervention target for neuroblastoma therapy. BMC Cancer 12:285 (2012).
Yes
