免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 重组兔单抗 > RAD18 Rabbit mAb

RAD18 Rabbit mAb#49044

评价
Recombinant
RAD18 Rabbit mAb
RAD18 Rabbit mAb
RAD18 Rabbit mAb
RAD18 Rabbit mAb
RAD18 Rabbit mAb
RAD18 Rabbit mAb
是否有货: Yes
产品总价:
产品详情

产品名称RAD18 Rabbit mAb

克隆号SN66-01

纯化ProA affinity purified

应用WB, ICC/IF, IHC, FC

种属反应性Hu, Ms, Rt

免疫原描述recombinant protein

别名2810024C04Rik antibody
DNA repair protein rad18 antibody
E3 ubiquitin-protein ligase RAD18 antibody
EC 6.3.2.- antibody
hHR 18 antibody
hHR18 antibody
hRAD 18 antibody
hRAD18 antibody
MGC156682 antibody
Postreplication repair E3 ubiquitin-protein ligase RAD18 antibody
Postreplication repair protein hRAD18p antibody
Postreplication repair protein RAD18 antibody
RAD 18 antibody
RAD18 antibody
RAD18 homolog (S. cerevisiae) antibody
RAD18 homolog antibody
RAD18 S. cerevisiae homolog antibody
RAD18 S. cerevisiae homolog of antibody
RAD18 transcript variant antibody
RAD18_HUMAN antibody
Rad18sc antibody
Radiation sensitivity protein 18 antibody
RING finger protein 73 antibody
RNF 73 antibody
RNF73 antibody
Structural maintenance of chromosomes protein 6 antibody
YCR066W antibody
YCR66W antibody

数据库入口号Swiss-Prot#:Q9NS91

UniprotQ9NS91

Gene ID56852

计算分子量56/90kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情

WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:50-1:100

Western blot analysis of RAD18 on different lysates using anti-RAD18 antibody at 1/1,000 dilution. Positive control:
Lane 1: NIH/3T3
Lane 2: 293T
Lane 3: MCF-7
Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RAD18 antibody. Counter stained with hematoxylin.
ICC staining RAD18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining RAD18 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining RAD18 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with RAD18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
背景

The RING-type zinc finger protein Rad18 is essential in post-replication repair of damaged DNA and contributes to the maintenance of genomic stability. Rad18 maintains chromosomal DNA with the Rad54-dependent DNA repair pathway and recruits ubiquitin-conjugating enzymes in the Rad6 pathway. Rad18 functions in gap-filling of a daughter strand on replication of damaged DNA and is localized in the nucleus.

背景文献

1. Meng X et al. Genetic Deficiency of Mtdh Gene in Mice Causes Male Infertility via Impaired Spermatogenesis and Alterations in the Expression of Small Non-coding RNAs. J Biol Chem 290:11853-64 (2015).
2. Shoaib M et al. PUB-NChIP--in vivo biotinylation" approach to study chromatin in proximity to a protein of interest. Genome Res 23:331-40 (2013).,,,, 151953,151953,49045,,MMP11 Rabbit mAb,Rabbit,Monoclonal,SN74-08,,ProA affinity purified,"WB, ICC/IF, IHC

如果您使用该产品49044发表了文章,请通知我们,让我们可以引用您的文献。

5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com