产品详情
产品名称MAP1LC3A Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号SN07-40
纯化ProA affinity purified
应用WB, ICC, IHC, IP
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名ATG8E antibody Autophagy-related protein LC3 A antibody Autophagy-related ubiquitin-like modifier LC3 A antibody LC3 antibody LC3A antibody MAP1 light chain 3 like protein 1 antibody MAP1 light chain 3-like protein 1 antibody MAP1A/1B light chain 3 A antibody MAP1A/MAP1B LC3 A antibody MAP1A/MAP1B light chain 3 A antibody MAP1ALC3 antibody MAP1BLC3 antibody Map1lc3a antibody Microtubule associated proteins 1A/1B light chain 3 antibody Microtubule-associated protein 1 light chain 3 alpha antibody Microtubule-associated proteins 1A and 1B, light chain 3 antibody Microtubule-associated proteins 1A/1B light chain 3A antibody MLP3A_HUMAN antibody
数据库入口号Swiss-Prot#:Q9H492
计算分子量14 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of MAP1LC3A on mouse brain lysates using anti-MAP1LC3A antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.
ICC staining MAP1LC3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MAP1LC3A in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3β) and MAP-light chain 3 alpha (MAP-LC3α), both of which are mammalian homologs of yeast Apg8, are subunits that can associate with either MAP-1A or MAP-1B. While MAP-LC3β is essential for autophagy and is associated with autophagosome membranes after processing, MAP LC3α is involved in the formation of autophagosomal vacuoles and is localized to the intracytoplasmic membrane. MAP LC3α is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver and skeletal muscle, but are absent in thymus and peripheral blood leukocytes.
背景文献
1. Koukourakis MI et al. Autophagosome Proteins LC3A, LC3B and LC3C Have Distinct Subcellular Distribution Kinetics and Expression in Cancer Cell Lines. PLoS One 10:e0137675 (2015). 2. Kim JY et al. Expression of autophagy-related proteins according to androgen receptor and HER-2 status in estrogen receptor-negative breast cancer. PLoS One 9:e105666 (2014).
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