产品详情
产品名称ABCF1 Rabbit mAb
克隆号SN07-12
纯化ProA affinity purified
应用WB, ICC/IF, IHC
种属反应性Hu, Rt
免疫原描述Synthetic peptide within aa 796-845 of Human ABCF1
别名ABC27 antibody ABC50 antibody ABCF1 antibody ABCF1_HUMAN antibody ATP binding cassette 50 (TNF alpha stimulated) antibody ATP binding cassette 50 antibody ATP binding cassette sub family F member 1 antibody ATP binding cassette, sub family F (GCN20), member 1 antibody ATP-binding cassette 50 antibody ATP-binding cassette sub-family F member 1 antibody AU041969 antibody D17Wsu166e antibody EST123147 antibody GCN20 antibody TNF alpha stimulated ABC protein antibody TNF-alpha-stimulated ABC protein antibody TNFalpha inducible ATP binding protein antibody wu:fb79c06 antibody wu:fc39a05 antibody wu:fj94a08 antibody zgc:85667 antibody
数据库入口号Swiss-Prot#:Q8NE71
计算分子量96 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500
Western blot analysis of ABCF1 on Hela lysates using anti-ABCF1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.
ICC staining ABCF1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ABCF1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ABCF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
ABCF1 (ATP-binding cassette sub-family F member 1, TNF-alpha-stimulated ABC protein) is a 845 amino acid protein encoded by the human gene ABCF1. ABCF1 belongs to the ABC transporter family (EF3 subfamily) and contains two ABC transporter domains. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. ABCF1 is believed to have a role in mRNA translation due to its interaction with eukaryotic initiation factor 2 (eIF2). It is also associated with ribosomes. ABCF1 is ubiquitously expressed and can be induced with TNF. Upon DNA damage, ABCF1 is phosphorylated by either ATM or ATR.
1. Pechenino AS et al. Impact of aging vs. estrogen loss on cardiac gene expression: estrogen replacement and inflammation. Physiol Genomics 43:1065-73 (2011).
2. DU, P. et al. Metastasis Suppressor-1, MTSS1, Acts as a Putative Tumour Suppressor in Human Bladder Cancer. Anticancer Res 31: 3205-3212 (2011).
Yes
