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位置: 首页 > 磷酸化抗体 > Akt2(Phospho-Ser474) Antibody

Akt2(Phospho-Ser474) Antibody#11124

Akt2(Phospho-Ser474) Antibody
Akt2(Phospho-Ser474) Antibody
Akt2(Phospho-Ser474) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Akt2(Phospho-Ser474) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Akt2 only when phosphorylated at serine 474.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 474 (Q-F-S(p)-Y-S) derived from Human Akt2.

基因/蛋白名称Akt2

修饰Phospho

别名PKB beta; Protein kinase B; RAC-PK-beta

数据库入口号Swiss-Prot: P31751
NCBI Protein: NP _001617.1

UniprotP31751

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using Akt2(Phospho-Ser474) Antibody #11124.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2(Phospho-Ser474) Antibody #11124(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from 293 cells, treated with serum or calf intestinal phosphatase (CIP), using Akt2 (Phospho-Ser474) Antibody #11124.
背景

General protein kinase capable of phosphorylating several known proteins.

Sun M, et al. (2001) Cancer Res; 61(16): 5985-91.
Yuan ZQ, et al. (2000) Oncogene; 19(19): 2324-30.
Meier R, et al. (1997) J Biol Chem; 272(48): 30491-7.

如果您使用该产品11124发表了文章,请通知我们,让我们可以引用您的文献。

et al,The effect of cyclic tensile force on the actin cytoskeleton organization and morphology of human periodontal ligament cells.In Biochem Biophys Res Commun.On 2018 Dec 2 by Wang YF, Zuo ZH et al..PMID: 30401563, , (2018),
PMID: 30401563
et al,Girdin/GIV is upregulated by cyclic tension, propagates mechanical signal transduction, and is required for the cellular proliferation and migration of MG-63 cells.In Biochem Biophys Res Commun.On 2015 Aug 21 by Hu JT, Li Y et al..PMID:26163263, , (2015),
PMID: 26163263
et al,ApoA5 knockdown improves whole-body insulin sensitivity in high-fat-fed mice by reducing ectopic lipid content.In J Lipid Res on 2015 Mar by Jo茫o Paulo G Camporez , Shoichi Kanda et al..PMID: 25548259, , (2015),
PMID: 25548259
et al,Targeting steroid receptor coactivator 1 with antisense oligonucleotides increases insulin-stimulated skeletal muscle glucose uptake in chow-fed and high-fat-fed male rats. In Am J Physiol Endocrinol Metab on 2014 Nov 1 by Jennifer L Cantley, Daniel F Vatner,et al..PMID:25159329, , (2014),
PMID: 25159329
et al,Differential apoptosis gene expressions of rhabdomyosarcoma cells in response to enterovirus 71 infection.In BMC Infect Dis on 2012 Nov 28 by Shi W, Li X,et al..PMID:23191987, , (2012),
PMID: 23191987
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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