产品详情
产品名称ATF4 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号SD20-92
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名Activating transcription factor 4 antibody
ATF 4 antibody
ATF4 antibody
ATF4 protein antibody
ATF4_HUMAN antibody
cAMP-dependent transcription factor ATF-4 antibody
cAMP-responsive element-binding protein 2 antibody
CREB 2 antibody
CREB-2 antibody
CREB2 antibody
Cyclic AMP dependent transcription factor ATF 4 antibody
Cyclic AMP response element binding protein 2 antibody
Cyclic AMP-dependent transcription factor ATF-4 antibody
Cyclic AMP-responsive element-binding protein 2 antibody
DNA binding protein TAXREB67 antibody
DNA-binding protein TAXREB67 antibody
Tax Responsive Enhancer Element B67 antibody
Tax-responsive enhancer element-binding protein 67 antibody
TaxREB67 antibody
TXREB antibody
数据库入口号Swiss-Prot#:P18848
计算分子量55 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of ATF4 on different lysates using anti-ATF4 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: PC-12
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ATF4 antibody. Counter stained with hematoxylin.
ICC staining ATF4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ATF4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ATF4 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with ATF4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promoter and enhancer elements. The cAMP response element (CRE), one of the best studied of such elements, consists of the palindromic octanucleotide TGACGTCA. Several CRE binding proteins have been identified within the ATF/CREB family, the best characterized of which include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. These proteins share highly related COOH terminal leucine zipper dimerization and basic DNA binding domains but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins appear capable of binding CRE in its homodimeric form, certain of these also bind as heterodimers, both within the ATF/CREB family and even with members of the AP-1 transcription factor family.
背景文献
1. Zhu H et al. Activating transcription factor 4 promotes esophageal squamous cell carcinoma invasion and metastasis in mice and is associated with poor prognosis in human patients. PLoS One 9:e103882 (2014).
2. Lenna S et al. HLA-B35 and dsRNA induce endothelin-1 via activation of ATF4 in human microvascular endothelial cells. PLoS One 8:e56123 (2013).
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et al,The therapeutic effect of tanshinone IIA in mouse astrocytes after treatment with Angiostrongylus cantonensis fifth-stage larval excretory-secretory productsIn J Microbiol Immunol Infect.On2023 AugbyKuang-Yao Chen , Yi-Ju Chen et al..PMID:37147244,
, (2023),
PMID: 37147244
et al,Achyranthes bidentata polysaccharides alleviate endoplasmic reticulum stress in osteoarthritis via lncRNA NEAT1/miR-377-3p pathway. In Biomed Pharmacother
on 2022 Oct by Changlong Fu, Zhiwei Qiu,et al..PMID:35988424,
, (2022),
PMID: 35988424
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