CD3D Rabbit mAb - SAB | Signalway Antibody

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CD3D Rabbit mAb#49268

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Recombinant

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产品详情

产品名称CD3D Rabbit mAb

克隆号JJ08-97

纯化Affinity-chromatography

应用WB;IHC;ICC/IF;IP;FC

种属反应性Hu

免疫原描述A synthesized peptide derived from human CD3 delta

别名CD3 antigen delta subunit antibody
CD3 delta antibody
CD3d antibody
CD3d antigen delta polypeptide (TiT3 complex) antibody
CD3d molecule delta (CD3-TCR complex) antibody
CD3D_HUMAN antibody
IMD19 antibody
OKT3 delta chain antibody
T cell receptor T3 delta chain antibody
T-cell receptor T3 delta chain antibody
T-cell surface glycoprotein CD3 delta chain antibody
T3D antibody

数据库入口号Swiss-Prot#:P04234

计算分子量19 kDa

配方Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

应用详情

WB 1:1000-1:2000
IHC 1:100-1:200
ICC/IF 1:50-1:200
IP 1:20-1:50
FC 1:20-1:100

Flow cytometric analysis of Raji cells with CD3D antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human spleen, using CD3D Antibody.

Immunofluorescent analysis using the Antibody at 1:50 dilution.

Immunofluorescent analysis of Jurkat cells, using CD3D Antibody.

背景

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response.

背景文献

1. Li X., et al. 2015. Identification of a FOXP3(+)CD3(+)CD56(+) population with immunosuppressive function in cancer tissues of human hepatocellular carcinoma. Sci Rep 5:14757-14757.
2. He Y., et al. 2015. Identification of the Docking Site for CD3 on the T Cell Receptor beta Chain by Solution NMR. J. Biol. Chem. 290:19796-19805.

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操作手册

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应用

WB免疫印迹
IHC免疫组化
IF免疫荧光
ICC免疫细胞化学
FC流式细胞
IP免疫沉淀
E酶联免疫吸附法
DB免疫斑点法
ChIP染色质免疫沉淀
GICA胶体金免疫层析法
NC阴性对照

种属反应性

Hu人
Ms小鼠
Rt大鼠
Dm果蝇
C线虫
Mk猴
Rb兔
B牛
D狗
P猪
Hm仓鼠
ChHm中国仓鼠
Chk鸡

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CD3D Rabbit mAb#49268

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CD3D Rabbit mAb#49268

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