产品详情
产品名称TSG101 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JJ0900
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名ESCRT I complex subunit TSG101 antibody ESCRT-I complex subunit TSG101 antibody TS101_HUMAN antibody TSG 10 antibody TSG 101 antibody TSG10 antibody Tsg101 antibody Tumor susceptibility gene 10 antibody Tumor susceptibility gene 101 antibody Tumor susceptibility gene 101 protein antibody Tumor susceptibility protein antibody Tumor susceptibility protein isoform 3 antibody VPS 23 antibody VPS23 antibody
数据库入口号Swiss-Prot#:Q99816
计算分子量44 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC: 1:50-1:100
Western blot analysis of TSG101 on different lysates using anti-TSG101 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Hela Lane 3: K562 Lane 4: Jurkat Lane 5: A431
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TSG101 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TSG101 antibody. Counter stained with hematoxylin.
ICC staining TSG101 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining TSG101 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining TSG101 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of K562 cells with TSG101 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
The transformation of a normal cell to one that is malignant can result from mutations in genes that encode proteins with key regulatory functions. Examples include the retinoblastoma gene product (Rb p110), p53, VHL and APC. Using a novel cloning strategy that allows the isolation of previously uncharacterized genes encoding selectable recessive phenotypes, an additional tumor suppressor gene has been identified. This gene, termed tsg 101 for tumor susceptibility gene 101, encodes a stathmin binding domain protein. When expression of this growth inhibitory gene is blocked in NIH/3T3 cells using antisense mRNA, the cells exhibit a transformed phenotype and are tumorigenic in SL6 mice.
背景文献
1. Ruiz-Guillen M et al. Capsid-deficient alphaviruses generate propagative infectious microvesicles at the plasma membrane. Cell Mol Life Sci 73:3897-916 (2016). 2. Baranyai T et al. Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods. PLoS One 10:e0145686 (2015).
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et al,Differential traits between microvesicles and exosomes in enterovirus infectionInMedCommOn2023 Sep 24byYuxuan Fu?1,?Sidong Xiong et al..PMID: 37752943,
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, (2022),
PMID: 35643547
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