产品详情
产品名称67kDa Laminin Receptor Rabbit mAb
克隆号JF0955
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名34/67 kDa laminin receptor antibody 37 kDa laminin receptor precursor antibody 37/67 kDa laminin receptor antibody 37LRP antibody 40S ribosomal protein SA antibody 67 kDa laminin receptor antibody 67LR antibody Colon carcinoma laminin binding protein antibody Colon carcinoma laminin-binding protein antibody LAMBR antibody Laminin receptor 1 antibody Laminin-binding protein precursor p40 antibody LAMR 1 antibody LamR antibody LAMR1 antibody LBP antibody LBP/p40 antibody LRP antibody LRP/LR antibody Multidrug resistance associated protein MGr1 Ag antibody Multidrug resistance associated protein MGr1Ag antibody Multidrug resistance-associated protein MGr1-Ag antibody NEM/1CHD4 antibody p40 antibody Ribosomal Protein SA antibody rpsA antibody RSSA_HUMAN antibody SA antibody
数据库入口号Swiss-Prot#:P08865
计算分子量40 kDa
浓度1 mg/ml
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC: 1:50-1:100
Western blot analysis of RPSA on different lysates using anti-RPSA antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: HepG2 Lane 3: A431
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-RPSA antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-RPSA antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-RPSA antibody. Counter stained with hematoxylin.
ICC staining RPSA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining RPSA in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining RPSA in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining RPSA in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of MCF-7 cells with RPSA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
Laminin receptor (Laminin-R) has a heterodimeric structure similar to that of receptors for other extracellular matrix proteins such as Fibronectin and Vitronectin. Incorporation of Laminin-R into lysosomal membranes makes it possible for lysosomes to attach to surfaces coated with Laminin. This and other properties identify Laminin-R as a member of the integrin family of cell adhesion receptors. The Laminin-R precursor is a polypeptide whose expression is consistently upregulated in aggressive carcinoma. The precursor, which is also identified as p40 ribosome-associated protein, appears to be a multifunctional protein involved in the translational machinery. Laminin-R (also known as colon carcinoma laminin-binding protein) and is found at nine-fold higher levels in colon carcinoma than in adjacent normal colonic epithelium. Additionally, the level of the Laminin-R is higher in the lung cancer cell line than in the lung cell line.
1. Conway MJ et al. Mosquito saliva serine protease enhances dissemination of dengue virus into the mammalian host. J Virol 88:164-75 (2014).
2. K��hne H et al. Vitamin D receptor regulates intestinal proteins involved in cell proliferation, migration and stress response. Lipids Health Dis 13:51 (2014).
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