免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 重组兔单抗 > CD74 Rabbit mAb

CD74 Rabbit mAb#49365

评价
Recombinant
CD74 Rabbit mAb
CD74 Rabbit mAb
CD74 Rabbit mAb
CD74 Rabbit mAb
CD74 Rabbit mAb
CD74 Rabbit mAb
CD74 Rabbit mAb
是否有货: Yes
产品总价:
产品详情

产品名称CD74 Rabbit mAb

克隆号JF40-10

纯化Affinity-chromatography

应用WB, ICC/IF, IHC, FC

种属反应性Hu

免疫原描述A synthesized peptide derived from human CD74

别名CD 74 antibody
CD74 antibody
CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
CD74 antigen antibody
CD74 molecule antibody
CD74 molecule, major histocompatibility complex, class II invariant chain antibody
CLIP antibody
DHLAG antibody
Gamma chain of class II antigens antibody
HG2A_HUMAN antibody
HLA class II histocompatibility antigen gamma chain antibody
HLA DR antigens associated invariant chain antibody
HLA DR gamma antibody
HLA-DR antigens-associated invariant chain antibody
HLA-DR-gamma antibody
HLADG antibody
HLADR antigens associated invariant chain antibody
Ia antigen associated invariant chain antibody
Ia antigen-associated invariant chain antibody
Ia associated invariant chain antibody
Ia gamma antibody
Ii antibody
Invariant polypeptide of major histocompatibility complex class II antigen associated antibody
la-gamma antibody
Major histocompatibility complex class II invariant chain antibody
MHC HLA DR gamma chain antibody
MHC HLA-DR gamma chain antibody
p33 antibody
p35 antibody
Protein 41 antibody

数据库入口号Swiss-Prot#:P04233

计算分子量34 kDa

配方Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

应用详情

WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100

Western blot analysis of CD74 on Raji cells lysates using anti-CD74 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD74 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD74 antibody. Counter stained with hematoxylin.
ICC staining CD74 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD74 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD74 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Jurkat cells with CD74 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景

The human histocompatibility leukocyte antigen (HLA) class II-associated invariant chain is composed of at least four polypeptides. One of these polypeptide chains is expressed as a membrane-bound subunit and has been designated CD74. The loading of peptide onto the class II MHC protein (MHC II) appears to be regulated by CD74, which associates with MHC II during its migration to the endosomal compartment, where class II binds pep-tide. CD74 is expressed by cells of both T lymphocyte and B lymphocyte lineages. In fact, CD74 is broadly expressed in normal B lymphocytes, regardless of their histocompatibility leukocyte antigen (HLA) phenotype, while a subset of peripheral T lymphocytes that are MHC II negative do not express CD74.

背景文献

1. Piao Y et al. Acquired resistance to anti-VEGF therapy in glioblastoma is associated with a mesenchymal transition. Clin Cancer Res 19:4392-403 (2013).
2. Fernandez-Cuesta L et al. CD74-NRG1 Fusions in Lung Adenocarcinoma. Cancer Discov 4:415-22 (2014).

如果您使用该产品49365发表了文章,请通知我们,让我们可以引用您的文献。

5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com