产品详情
产品名称DDIT3 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JM10-31
纯化ProA affinity purified
应用WB, IHC, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
别名C/EBP homologous protein antibody C/EBP Homology Protein antibody C/EBP zeta antibody C/EBP-homologous protein 10 antibody C/EBP-homologous protein antibody CCAAT/enhancer binding protein homologous protein antibody CEBPZ antibody CHOP 10 antibody CHOP antibody CHOP-10 antibody CHOP10 antibody DDIT 3 antibody DDIT-3 antibody Ddit3 antibody DDIT3_HUMAN antibody DNA Damage Inducible Transcript 3 antibody DNA damage-inducible transcript 3 protein antibody GADD 153 antibody GADD153 antibody Growth Arrest and DNA Damage Inducible Protein 153 antibody Growth arrest and DNA damage inducible protein GADD153 antibody Growth arrest and DNA damage-inducible protein GADD153 antibody MGC4154 antibody
数据库入口号Swiss-Prot#:P35638
计算分子量25 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000
IHC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of DDIT3 on PC-12 cells lysates using anti-DDIT3 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Hela cells with DDIT3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
GADD 153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. This protein has also been designated C/EBP-homologous protein (CHOP-10). GADD 153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and metabolic perturbations. GADD 153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD 153 dimers away from "classical" C/EBP binding sites, recognizing instead unique "nonclassical" sites. Thus GADD 153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells, similar to the regulatory function of Id on the activity of Myo D and Myo D-related proteins involved in the development of muscle cells.
背景文献
1. Sun XY et al. Valproate attenuates diabetic nephropathy through inhibition of endoplasmic reticulum stress-induced apoptosis. Mol Med Rep 13:661-8 (2016). 2. Greenwood M et al. Transcription Factor CREB3L1 Regulates Endoplasmic Reticulum Stress Response Genes in the Osmotically Challenged Rat Hypothalamus. PLoS One 10:e0124956 (2015).
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