产品详情
产品名称Vitamin D Binding protein Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JM10-36
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC
种属反应性Hu
免疫原描述recombinant protein
别名DBP antibody
DBP/GC antibody
GC antibody
Gc globulin antibody
Gc-globulin antibody
GRD3 antibody
Group specific component antibody
Group specific component vitamin D binding protein antibody
Group-specific component antibody
hDBP antibody
VDB antibody
VDBG antibody
VDBP antibody
Vitamin D binding alpha globulin antibody
Vitamin D-binding protein antibody
VTDB_HUMAN antibody
数据库入口号Swiss-Prot#:P02774
计算分子量53 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of DBP on human lung lysates using anti- DBP antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- DBP antibody. Counter stained with hematoxylin.
ICC staining DBP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining DBP in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining DBP in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of HepG2 cells with DBP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
Vitamin D-binding protein (DBP) is a multi-functional serum protein that binds to the plasma membranes of numerous cell types and mediates a variety of cellular functions. The locus of the DBP protein (also known as group-specific component protein or GC) is located at human chromosome 4q13.3. DBP functions in organ-specific transportation of vitamin D and its metabolites to the various target organs of the vitamin D endocrine system. In addition, DBP has immunomodulatory properties and is able to bind to the surface of leukocytes. DBP binds to the plasma membrane through a chondroitin sulfate proteoglycan. DBP serves as a co-chemotactic factor for C5a to enhance the chemotactic activity of C5a. DBP can also bind to globular Actin with high affinity and is involved in the clearance of Actin from the blood. DBP plays an important role in osteoclast differentiation. The diverse cellular functions of DBP require its cell surface binding ability to mediate different biological processes.
背景文献
1. Ackermann AM et al. Integration of ATAC-seq and RNA-seq identifies human alpha cell and beta cell signature genes. Mol Metab 5:233-44 (2016).
2. Tao W et al. Hormonal induction and roles of Disabled-2 in lactation and involution. PLoS One 9:e110737 (2014).
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