产品详情
产品名称Lipocalin-2 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JM10-67
纯化ProA affinity purified
应用WB, ICC/IF, IHC
种属反应性Hu
免疫原描述recombinant protein
别名24p3 antibody
25 kDa alpha 2 microglobulin related subunit of MMP9 antibody
25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody
Alpha 2 microglobulin related protein antibody
HGNC:6526 antibody
HNL antibody
Lcn 2 antibody
Lcn2 antibody
Lipocalin-2 antibody
Migration stimulating factor inhibitor antibody
MSFI antibody
Neutrophil gelatinase associated lipocalin antibody
Neutrophil gelatinase associated lipocalin precursor antibody
Neutrophil gelatinase-associated lipocalin antibody
NGAL antibody
NGAL_HUMAN antibody
Oncogene 24p3 antibody
Oncogenic lipocalin 24p3 antibody
p25 antibody
Siderocalin antibody
siderocalin LCN2 antibody
SV40 induced 24P3 protein antibody
Uterocalin antibody
数据库入口号Swiss-Prot#:P80188
计算分子量23 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC: 1:50-1:100
Western blot analysis of Lipocalin-2 on A431 cells lysates using anti-Lipocalin-2 antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Lipocalin-2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Lipocalin-2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Lipocalin-2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Lipocalin-2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Lipocalin-2 antibody. Counter stained with hematoxylin.
ICC staining Lipocalin-2 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Lipocalin-2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Lipocalin-2 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
In addition to the monomeric mammalian progelatinase, two additional forms of progelatinase have been identified. The shorter of these additional forms is a covalently linked, disulfide-bridged protein that heterodimerizes with a short protein; an α2-Microglobulin-related protein known as neutrophil gelatinase-associated lipocalin (NGAL), which is moderately expressed in breast and lung tissues. NGAL belongs to the lipocalin family and has a high degree of similarity with rat α2-Microglobulin-related protein and mouse protein 24p3. NGAL is able to bind a derivative of the bacterial chemotactic peptide, suggesting that it has important immuno-modulatory functions. NGAL has been described as an inflammatory protein; it is released into the circulation as a result of the inflammatory activation of leukocytes initiated by the extra-corporeal circulation. In addition, NGAL synthesis is induced in epithelial cells in inflammatory and neoplastic colorectal diseases. In conclusion, NGAL may serve as a scavenger of bacterial products to function in the anti-inflammatory process.
背景文献
1. Wolk K et al. Deficient cutaneous antibacterial competence in cutaneous T-cell lymphomas: role of Th2-mediated biased Th17 function. Clin Cancer Res 20:5507-16 (2014). 2. Chung TW et al. Lipocalin-2 elicited by advanced glycation end-products promotes the migration of vascular smooth muscle cells. Biochim Biophys Acta 1833:3386-95 (2013).
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