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位置: 首页 > 重组兔单抗 > VCP Rabbit mAb

VCP Rabbit mAb#49469

评价
Recombinant
VCP Rabbit mAb
VCP Rabbit mAb
VCP Rabbit mAb
VCP Rabbit mAb
VCP Rabbit mAb
VCP Rabbit mAb
VCP Rabbit mAb
VCP Rabbit mAb
VCP Rabbit mAb
VCP Rabbit mAb
是否有货: Yes
产品总价:
产品详情

产品名称VCP Rabbit mAb

来源种属Recombinant Rabbit

克隆性 Monoclonal antibody

克隆号JM11-15

纯化ProA affinity purified

应用WB, ICC/IF, IHC, FC, IP

种属反应性Hu, Ms, Rt, Zebrafish

免疫原描述recombinant protein

别名15S Mg(2+) ATPase p97 subunit antibody
15S Mg(2+)-ATPase p97 subunit antibody
ALS14 antibody
ATPase p97 antibody
CDC48 antibody
IBMPFD antibody
MGC131997 antibody
MGC148092 antibody
MGC8560 antibody
p97 antibody
TER ATPase antibody
TERA antibody
TERA_HUMAN antibody
Transitional endoplasmic reticulum ATPase antibody
Valosin containing protein antibody
Valosin-containing protein antibody
VCP antibody
Yeast Cdc48p homolog antibody

数据库入口号Swiss-Prot#:P55072

计算分子量89 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of VCP on different cells lysates using anti-VCP antibody at 1/500 dilution. Positive control�� Line 1: SH-SY5Y Line 2: HepG2 Line 3: Hela
Western blot analysis of VCP on hybrid fish (crucian-carp) heart tissue lysate using anti-VCP antibody at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-VCP antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VCP antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-VCP antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-VCP antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VCP antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-VCP antibody. Counter stained with hematoxylin.
ICC staining VCP in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VCP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Valosin containing protein (VCP), also designated TERA (for transitional endoplasmic reticulum ATPase) or p97, is a member of the AAA family of ATPases, which are involved in a variety of cellular activities. VCP is the mammalian homolog of Saccharomyces cerevisiae Cdc48, a protein essential for the completion of mitiosis in yeast. VCP is thought to be involved in a variety of membrane functions and in the regulation of the cell cycle. It associates with ubiquitinated IκB-α as well as with the 26S Proteosome, indicating a potential role for VCP in the proteosome-mediated degradation of IκB-α.
背景文献
1. Guo X et al. VCP recruitment to mitochondria causes mitophagy impairment and neurodegeneration in models of Huntington's disease. Nat Commun 7:12646 (2016).
2. Franz A et al. Chromatin-associated degradation is defined by UBXN-3/FAF1 to safeguard DNA replication fork progression. Nat Commun 7:10612 (2016).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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