产品详情
产品名称Epac1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, IHC, IP, ICC/IF
种属反应性Hu, Ms
免疫原描述Recombinant protein
别名bcm910 antibody
CAMP GEFI antibody
cAMP regulated guanine nucleotide exchange factor I antibody
CAMPGEFI antibody
CGEF 1 antibody
CGEF1 antibody
EPA1 antibody
Epac 1 antibody
EPAC antibody
EPAC1 antibody
Exchange factor directly activated by cAMP 1 antibody
Exchange protein directly activated by cAMP 1 antibody
MGC21410 antibody
RAP guanine nucleotide exchange factor antibody
Rap guanine nucleotide exchange factor (GEF) 3 antibody
RAP guanine nucleotide exchange factor 3 antibody
Rap1 guanine nucleotide exchange factor directly activated by cAMP antibody
RAPGEF3 antibody
数据库入口号Swiss-Prot#:O95398
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC/IF: 1:10-1:50
IP: 1:10-1:50
Western blot analysis of Epac1 on mouse kidney tissue lysate using anti-Epac1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Epac1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Epac1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Epac1 antibody. Counter stained with hematoxylin.
背景
3',5' cyclic adenosine monophosphate (cAMP)-regulated guanine nucleotide exchange factors Epac (Epac1, cAMP-GEFI) and Epac2 (cAMP-GEFII) activate the Ras family GTPases Rap 1 and Rap 2 by promoting GTP binding in a cAMP-dependent manner. Eukaryotic cAMP is a second messenger that induces physiological responses such as gene expression, growth, differentiation, secretion and neurotransmission. The human Epac gene maps to chromosome 12q13.11 with transcript being abundant in the kidney and heart. In situ hybridization indicates expression of Epac in adult rat brain and selective expression in neonatal brain, including septum and thalamus.
背景文献
1. Guibinga GH et al. Deficiency of the purine metabolic gene HPRT dysregulates microRNA-17 family cluster and guanine-based cellular functions: a role for EPAC in Lesch-Nyhan syndrome. Hum Mol Genet 22:4502-15 (2013).
2. Yi L et al. TLR signaling paralyzes monocyte chemotaxis through synergized effects of p38 MAPK and global Rap-1 activation. PLoS One 7:e30404 (2012).
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