产品详情
产品名称Vinculin Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JM42-43
纯化ProA affinity purified
应用WB, ICC/IF, IP
种属反应性Hu, Ms, Rt
免疫原描述Recombinant protein
别名CMD1W antibody
CMH15 antibody
Epididymis luminal protein 114 antibody
HEL114 antibody
Metavinculin antibody
MV antibody
MVCL antibody
OTTHUMP00000019861 antibody
OTTHUMP00000019862 antibody
VCL antibody
VINC antibody
VINC_HUMAN antibody
Vinculin antibody
数据库入口号Swiss-Prot#:P18206
计算分子量124 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
ICC/IF: 1:50-1:200
IP: 1:10-1:50
Western blot analysis of Vinculin on different lysates using anti-Vinculin antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane2: Human fetal skeletal muscle Lane 3: Rat spleen
Lane 4: Mouse kidney
ICC staining Vinculin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Vinculin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Vinculin in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and α-actinin. In addition, vinculin, Talin and α-actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. α-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
背景文献
1. King HO et al. RAD51 Is a Selective DNA Repair Target to Radiosensitize Glioma Stem Cells. Stem Cell Reports 8:125-139 (2017).
2. Boyette LB et al. Phenotype, function, and differentiation potential of human monocyte subsets. PLoS One 12:e0176460 (2017).
Yes
