产品详情
产品名称Zyxin Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JU83-12
纯化ProA affinity purified
应用WB,ICC/IF,IHC,FC,IP
种属反应性Hu, Ms
免疫原描述Recombinant protein
别名ESP 2 antibody
ESP2 antibody
HED 2 antibody
HED2 antibody
ZYX antibody
ZYX protein antibody
Zyxin 2 antibody
Zyxin2 antibody
数据库入口号Swiss-Prot#:Q15942
计算分子量75/61/45 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of Zyxin on MCF-7 cell using anti-Zyxin antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Zyxin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Zyxin antibody. Counter stained with hematoxylin.
ICC staining Zyxin in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Zyxin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Zyxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
Zyxin is a low abundance phosphoprotein localized to focal adhesion plaques and is thought to perform regulatory functions at these regions. The protein contains a number of proline-rich sequences as well as three LIM domains, zinc finger domains involved in protein binding. Zyxin interacts with several other proteins at cell adhesion sites, including members of the CRP (cysteine-rich protein) LIM domain containing protein family. The proline-rich domain of Zyxin associates with an SH3 domain of p95 Vav, but not with similar SH3 domains containing proteins such as GRB2 or PLCg. Zyxin has also been shown to interact with the focal adhesion protein VASP and may assist in the targeting of VASP to focal adhesions, microfilaments and membrane regions of high dynamic activity.
背景文献
1. Han X et al. Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules. Nat Commun 8:14639 (2017).
2. Qi L et al. Talin2-mediated traction force drives matrix degradation and cell invasion. J Cell Sci 129:3661-3674 (2016).
Yes
