产品详情
产品名称DDB1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JU32-35
纯化ProA affinity purified
应用WB,ICC/IF,IHC,IP
种属反应性Hu, Ms, Rt
免疫原描述Recombinant protein
别名Damage specific DNA binding protein 1 antibody
Damage-specific DNA-binding protein 1 antibody
DDB 1 antibody
DDB p127 subunit antibody
Ddb1 antibody
DDB1_HUMAN antibody
DDBa antibody
DNA damage binding protein 1 antibody
DNA damage-binding protein 1 antibody
DNA damage-binding protein a antibody
HBV X-associated protein 1 antibody
UV damaged DNA binding factor antibody
UV damaged DNA binding protein 1 antibody
UV DDB 1 antibody
UV DDB1 antibody
UV-damaged DNA-binding factor antibody
UV-damaged DNA-binding protein 1 antibody
UV-DDB 1 antibody
X associated protein 1 antibody
XAP 1 antibody
XAP-1 antibody
XAP1 antibody
Xeroderma pigmentosum group E complementing protein antibody
Xeroderma pigmentosum group E-complementing protein antibody
XPCe antibody
XPE antibody
XPE BF antibody
XPE binding factor antibody
XPE-BF antibody
XPE-binding factor antibody
数据库入口号Swiss-Prot#:Q16531
计算分子量127 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
IP: 1:10-1:50
Western blot analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution. Positive control:
Lane 1: HepG2
Lane 2: NIH-3T3
Lane 3: MCF-7
Lane 4: Rat kidney tissue
Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
ICC staining DDB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining DDB1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Damaged DNA binding protein (DDB) is a heterodimer composed of two subunits, p127 and p48, which are designated DDB1 and DDB2, respectively. The DDB heterodimer is involved in repairing DNA damaged by ultraviolet light. Specifically, DDB, also designated UV-damaged DNA binding protein (UV-DDB), xeroderma pigmentosum group E binding factor (XPE-BF) and hepatitis B virus X-associated protein 1 (XAP-1), binds to damaged cyclobutane pyrimidine dimers (CPDs). Mutations in the DDB2 gene are implicated as causes of xeroderma pigmentosum group E, an autosomal recessive disease in which patients are defective in nucleotide excision DNA repair. XPE is characterized by hypersensitivity of the skin to sunlight with a high frequency of skin cancer as well as neurologic abnormalities. The hepatitis B virus (HBV) X protein interacts with DDB1, which may mediate HBx transactivation.
背景文献
1. Yu C et al. CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation. Nat Commun 6:8017 (2015).
2. Tamayo AG et al. Histone monoubiquitination by Clock-Bmal1 complex marks Per1 and Per2 genes for circadian feedback. Nat Struct Mol Biol 22:759-66 (2015).
Yes
