产品详情
产品名称Drosha Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JU33-01
纯化ProA affinity purified
应用WB,ICC,IF,IHC,FC
种属反应性Hu
免疫原描述Recombinant protein
别名DROSHA antibody
Drosha double stranded RNA specific endoribonuclease antibody
Drosha ribonuclease type III antibody
Etohi2 antibody
HSA242976 antibody
Nuclear RNase III Drosha antibody
p241 antibody
Protein Drosha antibody
Putative protein p241 which interacts with transcription factor Sp1 antibody
Putative ribonuclease III antibody
RANSE3L antibody
Ribonuclease 3 antibody
Ribonuclease III antibody
Ribonuclease III nuclear antibody
Ribonuclease type III nuclear antibody
RibonucleaseIII antibody
RN 3 antibody
RN3 antibody
RNase 3 antibody
RNase III antibody
RNase3 antibody
RNASE3L antibody
RNaseIII antibody
RNASEN antibody
RNC_HUMAN antibody
数据库入口号Swiss-Prot#:Q9NRR4
计算分子量159 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of Drosha on Hela cell and SiHa cell lysates using anti-Drosha antibody at 1/500 dilution.
Western blot analysis of Drosha on K562 cell lysates using anti-Drosha antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Drosha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Drosha antibody. Counter stained with hematoxylin.
ICC staining Drosha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Drosha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Drosha in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Jurkat cells with Drosha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
背景
The ribonuclease III superfamily represents a structurally distinct group of double-strand-specific endonucleases with essential roles in RNA maturation, RNA decay, and gene silencing. Initial cleavage of microRNAs is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcripts (pri-miRNAs) in the nucleus. Human Drosha is a component of two multi-protein complexes. The larger complex contains multiple classes of RNA-associated proteins including RNA helicases, proteins that bind double-stranded RNA, novel heterogeneous nuclear ribonucleoproteins and the Ewing's sarcoma family of proteins. The smaller complex is composed of Drosha and the double-stranded-RNA-binding protein, DGCR8.
背景文献
1. Wei YB et al. Elevation of Il6 is associated with disturbed let-7 biogenesis in a genetic model of depression. Transl Psychiatry 6:e869 (2016).
2. Adam O et al. Role of miR-21 in the pathogenesis of atrial fibrosis. Basic Res Cardiol 107:278 (2012).
Yes
