产品详情
产品名称ZAP70 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JU08-39
纯化ProA affinity purified
应用WB,ICC,IHC,FC,IP
种属反应性Hu
免疫原描述Recombinant protein
别名70 kDa zeta associated protein antibody
70 kDa zeta-associated protein antibody
EC 2.7.10.2 antibody
FLJ17670 antibody
FLJ17679 antibody
Selective T cell defect antibody
SRK antibody
STD antibody
Syk related tyrosine kinase antibody
Syk-related tyrosine kinase antibody
Truncated ZAP kinase antibody
Tyrosine protein kinase ZAP70 antibody
Tyrosine-protein kinase ZAP-70 antibody
TZK antibody
ZAP 70 antibody
ZAP70 antibody
ZAP70_HUMAN antibody
Zeta chain associated protein kinase 70kD antibody
Zeta chain associated protein kinase 70kDa antibody
Zeta chain associated protein kinase 70kDa isoform 1 antibody
Zeta chain associated protein kinase 70kDa isoform 2 antibody
Zeta chain of T cell receptor associated protein kinase 70 antibody
Zeta chain TCR associated protein kinase 70kD antibody
Zeta chain TCR associated protein kinase 70kDa antibody
数据库入口号Swiss-Prot#:P43403
计算分子量70 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
IP: 1:10-1:50
FC: 1:50-1:100
Western blot analysis of ZAP70 on human thymus tissue lysate using anti-ZAP70 antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZAP70 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ZAP70 antibody. Counter stained with hematoxylin.
ICC staining ZAP70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ZAP70 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Jurkat cells with ZAP70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
背景
The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti α and β chains, the CD3 γ, δ and e chains and a ζ-containing homodimer or heterodimer. The disulfide-linked Ti α-β heterodimer is responsible for antigen recognition, but the short 5 amino acid cytoplasmic domains of Ti α and β are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and ζ subunits suggest a role in signal transduction. Of these, the ζ chain, which is expressed as either a homodimer or heterodimer, has a short extracellular domain of only 9 amino acids, but a larger 113 amino acid cytoplasmic domain. A tyrosine phosphoprotein, ZAP-70, has been identified that associates with z and undergoes tyrosine phosphorylation following TCR stimulation.
背景文献
1. Lupher ML et al. A novel phosphotyrosine-binding domain in the N-terminal transforming region of Cbl interacts directly and selectively with ZAP-70 in T cells. The Journal of Biological Chemistry 271 (39): 24063-8 (1996).
2. Han J et al. The SH3 domain-containing adaptor HIP-55 mediates c-Jun N-terminal kinase activation in T cell receptor signaling. The Journal of Biological Chemistry 278 (52): 52195-202 (2003).
Yes
