产品详情
产品名称DKC1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JU34-32
纯化ProA affinity purified
应用WB,ICC,IF,IHC
种属反应性Hu, Ms, Rt
免疫原描述Recombinant protein
别名CBF5 antibody
CBF5 homolog antibody
Cbf5p homolog antibody
DKC 1 antibody
DKC antibody
Dkc1 antibody
DKC1_HUMAN antibody
DKCX antibody
Dyskeratosis congenita 1 antibody
Dyskeratosis congenita 1 dyskerin antibody
Dyskerin antibody
H/ACA ribonucleoprotein complex subunit 4 antibody
NAP 57 antibody
NAP57 antibody
NOLA 4 antibody
NOLA4 antibody
Nopp140 associated protein of 57 kDa antibody
Nopp140-associated protein of 57 kDa antibody
Nucleolar protein family A member 4 antibody
Nucleolar protein NAP57 antibody
snoRNP protein DKC1 antibody
XAP 101 antibody
XAP101 antibody
数据库入口号Swiss-Prot#:O60832
计算分子量57 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:100
ICC: 1:50-1:200
Western blot analysis of DKC1 on A431 cell lysate using anti-DKC1 antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DKC1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-DKC1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DKC1 antibody. Counter stained with
ICC staining DKC1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells).
背景文献
1. Knight S W et al. X-linked dyskeratosis congenita is predominantly caused by missense mutations in the DKC1 gene. Am J Hum Genet 65:50-58 (1999).
2. Heiss N S et al. Gene structure and expression of the mouse dyskeratosis congenita gene, dkc1. Genomics 67:153-163 (2000).
Yes
