产品详情
产品名称EB3 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JU38-30
纯化ProA affinity purified
应用WB,ICC,IF,IHC,FC
种属反应性Hu, Ms, Rt
免疫原描述Recombinant protein
别名APC binding protein antibody
EB 3 antibody
EB1 protein family member 3 antibody
EB3 antibody
EBF 3 antibody
EBF3 antibody
EBF3 S antibody
End binding protein 3 antibody
End-binding protein 3 antibody
MAPRE 3 antibody
MAPRE3 antibody
MARE3_HUMAN antibody
Member 3 antibody
Microtubule Associated Protein antibody
Microtubule associated protein RP/EB family member 3 antibody
Microtubule-associated protein RP/EB family member 3 antibody
OTTHUMP00000200910 antibody
RP 3 antibody
RP/EB Family antibody
RP3 antibody
数据库入口号Swiss-Prot#:Q9UPY8
计算分子量32 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse brain tissue
Lane 2: K562
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-EB3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EB3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-EB3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-EB3 antibody. Counter stained with hematoxylin.
ICC staining EB3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining EB3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining EB3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SH-SY-5Y cells with EB3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
背景
EB1 (MAPRE2, microtubule-associated protein, RP/EB family, member 2, EB2, RP1) may influence tumorigenesis of colorectal cancers and proliferative control of normal cells. EB1 may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR. Colorectal cancer is caused by the pathologic transformation of normal colonic epithelium to an adenomatous polyp, which can become an invasive cancer. APC (adenomatous polyposis coli) is a tumor suppressor gene, the mutation of which is one of the earliest events in colorectal carcinogenesis. A majority of the mutations result in the loss of the carboxy terminus of APC. EB1 has been shown to bind to the carboxy terminal region of APC, which implicates EB1 in APC suppression of colonic cancer. EB1 overexpression may play a role in the development of human esophageal squamous cell carcinoma (ESCC) by affecting APC function and activating the beta-catenin/TCF pathway. EB3 is related to EB1 and likewise associates with the microtubule cytoskeleton. EB3 is expressed predominantly in the central nervous system and preferentially associates with APCL.
背景文献
1. Yang C et al. EB1 and EB3 regulate microtubule minus end organization and Golgi morphology. J Cell Biol 216:3179-3198 (2017).
2. Komarova Y et al. Mammalian end binding proteins control persistent microtubule growth. J Cell Biol 184:691-706 (2009).
Yes
