产品详情
产品名称VPS35 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JB33-82
纯化ProA affinity purified
应用WB,IHC,FC
种属反应性Hu, Ms
免疫原描述Recombinant protein
别名DKFZp434E1211 antibody
DKFZp434P1672 antibody
FLJ10752 antibody
FLJ13588 antibody
FLJ20388 antibody
hVPS35 antibody
Maternal embryonic 3 antibody
Maternal-embryonic 3 antibody
MEM 3 antibody
MEM3 antibody
PARK17 antibody
TCCCTA00141 antibody
Vacuolar protein sorting 35 (yeast) antibody
Vacuolar protein sorting 35 antibody
Vacuolar protein sorting 35 homolog antibody
Vacuolar protein sorting associated protein 35 antibody
Vacuolar protein sorting-associated protein 35 antibody
Vesicle protein sorting 35 antibody
VPS 35 antibody
VPS35 antibody
VPS35_HUMAN antibody
数据库入口号Swiss-Prot#:Q96QK1
计算分子量91 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:100-1:300
FC: 1:50-1:100
Western blot analysis of VPS35 on different lysates using anti-VPS35 antibody at 1/500 dilution.
Positive control:
Lane 1: PC-3M
Lane 2: U937
Lane 3: SH-SY-5Y
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VPS35 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VPS35 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-VPS35 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VPS35 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of 293T cells with VPS35 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
背景
Vacuolar sorting proteins (VPSs) are required for proper trafficking of endocytic and biosynthetic proteins to the vacuole and play an important role in the budding process of cells. VPS35 (vacuolar protein sorting 35), also known as MEM3, is the 796 amino acid human homolog of the S. cerevisiae Vps35 protein. Localized to the cytoplasm and to the peripheral membrane, VPS35 is an essential component of the retromer complex which is involved in retrieval of lysosomal enzyme receptors from endosomes to the trans-Golgi network. VPS35 is expressed ubiquitously with highest expression in heart, placenta, brain, testis, kidney, colon, ovary and spleen. In addition to its crucial role in the retromer complex, VPS35 is part of a subcomplex that is required to regulate transcytosis of the polymeric immunoglobulin receptor from the basolateral to the apical surface of epithelial cells and hepatocytes.
背景文献
1. Arighi C N et al. Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor. J Cell Biol 165:123-133 (2004) .
2. Verges M et al. The mammalian retromer regulates transcytosis of the polymeric immunoglobulin receptor. Nat Cell Biol 6:763-769 (2004).
Yes
