产品详情
产品名称DDX6 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JB41-32
纯化ProA affinity purified
应用WB,IHC
种属反应性Hu, Ms, Rt
免疫原描述Recombinant protein
别名ATP dependent RNA helicase DDX6 antibody
ATP dependent RNA helicase p54 antibody
DDX6 antibody
DEAD (Asp Glu Ala Asp) box polypeptide 6 antibody
DEAD box 6 antibody
DEAD box protein 6 antibody
DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RNA helicase 54kD) antibody
FLJ36338 antibody
HLR2 antibody
Oncogene RCK antibody
P54 antibody
Probable ATP-dependent RNA helicase DDX6 antibody
RCK antibody
数据库入口号Swiss-Prot#:P26196
计算分子量54 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:100
Western blot analysis of DDX6 on different lysates using anti-DDX6 antibody at 1/500 dilution.
Positive control:
Lane 1: Human colon
Lane 2: SH-SY-5Y
Lane 3: SiHa
Lane 4: K562
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-DDX6 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DDX6 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-DDX6 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DDX6 antibody. Counter stained with hematoxylin.
背景
RCK, also known as DDX6 and P54, is a member of the DEAD-box RNA helicase family of proteins, all of which share common protein motifs. Found in most tissues, RCK is an unwindase that exhibits ATP-dependent RNA un-winding activity, as well as the ability to decay RNA in the 5'-3' direction. In non-malignant cells, RCK is associated with all processes of normal RNA metabolism including splicing, export and translation initiation. Mutations in the gene encoding RCK can cause the protein to be overexpressed, changing its function to that of an oncogene that positively regulates the expression of genes involved in cell growth and proliferation. It is believed that, through its unwindase activity, the main function of RCK is to downregulate mRNA expression and maintain normal transcriptional levels within the cell.
背景文献
1. Fenger-Groen M et al. Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping. Mol Cell 20:905-915 (2005).
2. Hu G et al. A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy. Nat Cell Biol 17:930-942 (2015).
Yes
