产品详情
产品名称OGT Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JB44-39
纯化ProA affinity purified
应用WB,ICC,IF,IHC,FC
种属反应性Hu, Ms, Rt
免疫原描述Recombinant protein
别名FLJ23071 antibody
GlcNAc transferase antibody
HRNT1 antibody
MGC22921 antibody
O GlcNAc antibody
O GlcNAc transferase p110 subunit antibody
O GlcNAc transferase subunit p110 antibody
O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
O linked N acetylglucosamine (GlcNAc) transferase antibody
O linked N acetylglucosamine transferase 110 kDa subunit antibody
O-GlcNAc transferase subunit p110 antibody
O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
ogt antibody
OGT1_HUMAN antibody
UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
数据库入口号Swiss-Prot#:O15294
计算分子量117 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500
IHC: 1:50-1:200
ICC: 1:500-1:1,000
FC: 1:50-1:100
Western blot analysis of OGT on Hela (1) and SiHa (2) cell lysate using anti-OGT antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-OGT antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-OGT antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-OGT antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-OGT antibody. Counter stained with hematoxylin.
ICC staining OGT in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining OGT in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining OGT in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with OGT antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
背景
O-linked N-acetylglucosamine (O-GlcNAc) transferase (also designated OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. O-GlcNAc transferase has been purified from rat liver. It exists as a heterotrimeric complex with two subunits of the same molecular mass and one shorter subunit. Both polypeptides are related; the short subunit band is either a proteolytic product of the polypeptide or the product of an alternative translation start site. O-GlcNAc transferase is expressed as multiple transcripts that are present in different amounts in various human tissues, with the highest levels of expression in pancreas. Immunofluorescence of human cells expressing rat O-GlcNAc transferase indicated that it is present in both the nucleus and cytosol. HeLa cells expressing O-GlcNAc transferase do not survive well during prolonged incubations, suggesting that this protein may be toxic to the cells.
背景文献
1. Yang X et al. Recruitment of O-GlcNAc transferase to promoters by corepressor mSin3A: coupling protein O-GlcNAcylation to transcriptional repression. Cell 110:69-80 (2002).
2. Yang X et al. Phosphoinositide signalling links O-GlcNAc transferase to insulin resistance. Nature 451:964-969 (2008).
Yes
