产品详情
产品名称Dcp1a Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JB51-34
纯化ProA affinity purified
应用WB,ICC,IHC,FC
种属反应性Hu
别名DCP1 decapping enzyme homolog A antibody
Dcp1a antibody
DCP1A_HUMAN antibody
Decapping enzyme hDcp1a antibody
Decapping mRNA 1A antibody
HSA275986 antibody
mRNA decapping enzyme 1A antibody
mRNA-decapping enzyme 1A antibody
Nbla00360 antibody
Putative protein product of Nbla00360 antibody
Smad4 interacting transcriptional co activator antibody
Smad4-interacting transcriptional co-activator antibody
Smad4-interacting transcriptional co-activator antibody
SMAD4IP1 antibody SMIF antibody
Transcription factor SMIF antibody
数据库入口号Swiss-Prot#:Q9NPI6
计算分子量63 kDa(Predicted band size)
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of Dcp1a on human liver tissue lysates using anti-Dcp1a antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Dcp1a antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Dcp1a antibody. Counter stained with hematoxylin.
ICC staining Dcp1a in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Dcp1a in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Dcp1a in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of LOVO cells with Dcp1a antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
背景
Cleavage of the 5'-cap structure is involved in the major 5'-to-3' and nonsense-mediated mRNA decay pathways. The protein complex consisting of Dcp1 and Dcp2 has been identified as the species responsible for the decapping reaction in Saccharomyces cerevisiae. In nonsense-mediated decay, the human decapping complex, made up of S. cerevisiae homologs Dcp1a and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. hDcp2 specifically hydrolyzes methylated capped RNA to release m(7)GDP, thereby aiding in mRNA degradation. Both Dcp1a and hDcp2 colocalize in the cytoplasm. In addition, Dcp1a interacts with Smad4 forming a complex with TGFβ and BMP-4. Dcp1a and Smad4 interact directly through a EVH1/WH1 domain on Dcp1a and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of Dcp1a as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of Dcp1a eliminates TGFβ-induced nuclear translocation of Dcp1a.
背景文献
1. Lykke-Andersen J. Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay. Mol Cell Biol 22:8114-8121 (2002).
2. Bai R-Y et al. SMIF, a Smad4-interacting protein that functions as a co-activator in TGFbeta signalling. Nat Cell Biol 4:181-190 (2002).
Yes
