产品详情
产品名称HIF-2 alpha Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JB24-42
纯化ProA affinity purified
应用WB,IHC,FC
种属反应性Hu, Ms, Rt
免疫原描述Recombinant protein
别名Basic helix loop helix PAS protein MOP2 antibody
Basic-helix-loop-helix-PAS protein MOP2 antibody
bHLHe73 antibody
Class E basic helix-loop-helix protein 73 antibody
ECYT4 antibody
Endothelial PAS domain containing protein 1 antibody
Endothelial pas domain protein 1 antibody
Endothelial PAS domain-containing protein 1 antibody
EPAS 1 antibody
EPAS-1 antibody
EPAS1 antibody
EPAS1_HUMAN antibody
HIF 1 alpha like factor antibody
HIF 2 alpha antibody
HIF-1-alpha-like factor antibody
HIF-2-alpha antibody
HIF2-alpha antibody
HIF2A antibody
HLF antibody
Hypoxia inducible factor 2 alpha antibody
Hypoxia inducible factor 2 alpha subunit antibody
Hypoxia-inducible factor 2-alpha antibody
Member of PAS protein 2 antibody
Member of pas superfamily 2 antibody
MOP 2 antibody
MOP2 antibody
PAS domain-containing protein 2 antibody
PASD2 antibody
数据库入口号Swiss-Prot#:Q99814
计算分子量96 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
IHC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of HIF-2 alpha on SiHa cell lysate using anti-HIF-2 alpha antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HIF-2 alpha antibody. Counter stained with hematoxylin.
Flow cytometric analysis of HUVEC cells with HIF-2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
背景
Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia.
背景文献
1. Ema M et al. Molecular mechanisms of transcription activation by HLF and HIF1alpha in response to hypoxia: their stabilization and redox signal-induced interaction with CBP/p300. EMBO J 18:1905-1914 (1999).
2. Furlow P W et al. Erythrocytosis-associated HIF-2alpha mutations demonstrate a critical role for residues C-terminal to the hydroxylacceptor proline. J Biol Chem 284:9050-9058 (2009).
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et al,Roxadustat ameliorates experimental colitis in mice by regulating macrophage polarization through increasing HIF levelInBiochim Biophys Acta Gen SubjOn2023 Dec 27byGuiping Kong?1,?Hu Hua et al..PMID: 38158022,
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et al,Obstructive Sleep Apnea Affects Lacrimal Gland Function. In Invest Ophthalmol Vis Sci on 2022 Mar 2 by Shaopan Wang, Xin He,et al..PMID:35238868,
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PMID: 35238868
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