产品详情
产品名称eEF1A1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JB44-13
纯化ProA affinity purified
应用WB,ICC,IF,IHC,FC,IP
种属反应性Hu, Ms, Rt
免疫原描述Recombinant protein
别名CCS 3 antibody
CCS3 antibody
Cervical cancer suppressor 3 antibody
chunp6927 antibody
CTCL tumor antigen antibody
EE1A1 antibody
EEF 1 antibody
EEF1A antibody
eEF1A-1 antibody
EEF1A1 antibody
EF-1-alpha-1 antibody
EF-Tu antibody
EF1A antibody
EF1a like protein antibody
EF1A1_HUMAN antibody
Elongation factor 1 alpha subunit antibody
Elongation factor 1-alpha 1 antibody
Elongation factor Tu antibody
Eukaryotic elongation factor 1 A-1 antibody
Eukaryotic translation elongation factor 1 alpha 1 antibody
Eukaryotic translation elongation factor 1 alpha 1 like 14 antibody
Glucocorticoid receptor AF 1 specific elongation factor antibody
GRAF 1EF antibody
HNGC:16303 antibody
ik:tdsubc_2a3 antibody
ik:tdsubc_2b3 antibody
LENG7 antibody
Leukocyte receptor cluster (LRC) member 7 antibody
Leukocyte receptor cluster member 7 antibody
Prostate tumor inducing protein 1 antibody
PTI1 antibody
tdsubc_2a3 antibody
Translation elongation factor 1 alpha 1 like 14 antibody
wu:fa91c07 antibody
wu:fa94b03 antibody
wu:fi13b09 antibody
xx:tdsubc_2a3 antibody
xx:tdsubc_2b3 antibody
数据库入口号Swiss-Prot#:P68104
计算分子量50 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of eEF1A1 on different lysates using anti-eEF1A1 antibody at 1/500 dilution.
Positive control:
Lane 1: Rat brain tissue
Lane 2: Mouse skeletal muscle
Lane 3: Mouse cerebellum
Lane 4: Rat skin
Lane 5: Daudi
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin.
ICC staining eEF1A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining eEF1A1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining eEF1A1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of THP-1 cells with eEF1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
背景
The elongation factor-1 complex is composed of two subunits, EF-1 α1 (elongation factor 1-alpha 1) and EF-1 α2 (elongation factor 1-alpha 2), and is responsible for the delivery of aminoacyl tRNAs to the ribosome. EF-1 α1 is expressed predominately in brain, placenta, lung, liver, kidney and pancreas, while EF-1 α2 is highly expressed in heart, brain and skeletal muscle. Both EF-1 α1 and α2 localize to the nucleus and belong to the GTP-binding elongation factor family. The gene encoding EF-1 α2, which maps to human chromosome 20q13.3, may play a role in the development of ovarian cancer, while the EF-1 α1 gene, mapping to chromosome 6Q14.1, is commonly present as an autoantigen in patients with Felty syndrome. Felty syndrome is a disorder characterized by rheumatoid arthritis, a swollen spleen, decreased white blood cell count, and increased susceptibility to infection.
背景文献
1. Maruyama T et al. Txk, a member of the non-receptor tyrosine kinase of the Tec family, forms a complex with poly(ADP-ribose) polymerase 1 and elongation factor 1alpha and regulates interferon-gamma gene transcription in Th1 cells. Clin Exp Immunol 147:164-175 (2007).
2. Boratko A et al. Elongation factor-1A1 is a novel substrate of the protein phosphatase 1-TIMAP complex. Int J Biochem Cell Biol 69:105-113 (2015).
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