产品详情
产品名称ERp29 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JG34-42
纯化ProA affinity purified
应用WB,ICC,IF,IHC
种属反应性Hu, Ms, Rt
免疫原描述Recombinant protein within human ERp29 aa 1-150.
别名C12orf8 antibody
Chromosome 12 open reading frame 8 antibody
Endoplasmic reticulum lumenal protein ERp28 antibody
Endoplasmic reticulum protein 29 antibody
Endoplasmic reticulum protein 29 isoform 1 antibody
Endoplasmic reticulum protein ERp29 antibody
Endoplasmic reticulum resident protein 28 antibody
Endoplasmic reticulum resident protein 29 antibody
Endoplasmic reticulum resident protein 31 antibody
Epididymis secretory protein Li 107 antibody
ERp28 antibody
ERP29_HUMAN antibody
ERp31 antibody
HEL S 107 antibody
PDI-DB antibody
PDIA9 antibody
Protein disulfide isomerase family A member 9 antibody
数据库入口号Swiss-Prot#:P30040
计算分子量29 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of ERp29 on THP-1 cell using anti-ERp29 antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ERp29 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-ERp29 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERp29 antibody. Counter stained with hematoxylin.
ICC staining ERp29 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ERp29 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ERp29 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
ERp29 is a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide-isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms.
背景文献
1. Hughes G J et al. Human liver protein map: update 1993. Electrophoresis 14:1216-1222 (1993).
2. Bordoli M R et al. A secreted tyrosine kinase acts in the extracellular environment. Cell 158:1033-1044 (2014).