产品详情
产品名称ELMO1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JG34-59
纯化ProA affinity purified
应用WB,IHC,FC,IP
种属反应性Hu, Ms, Rt
免疫原描述Recombinant protein within human ELMO1 aa 500 to 700.
别名CED 12 antibody
Ced 12 homolog 1 antibody
Ced 12 homolog antibody
CED-12 antibody
CED12 antibody
Ced12 homolog 1 antibody
Ced12 homolog antibody
ELMO 1 antibody
ELMO-1 antibody
Elmo1 antibody
ELMO1_HUMAN antibody
Engulfment and cell motility 1 antibody
Engulfment and cell motility protein 1 antibody
KIAA0281 antibody
MGC126406 antibody
Protein ced-12 homolog antibody
数据库入口号Swiss-Prot#:Q92556
计算分子量84 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
IHC: 1:50-1:200
IP: 1:50-1:100
FC: 1:50-1:100
Western blot analysis of ELMO1 on mouse spleen tissue and Jurkat cell lysates using anti-ELMO1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-ELMO1 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-ELMO1 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Flow cytometric analysis of SH-SY-5Y cells with ELMO1 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
背景
Elmo (engulfment and cell motility) proteins share similarity to C. elegans CED-12. The C. elegans genes ced-2, ced-5, ced-10 and ced-12, and their mammalian homologs, CRKII, DOCK1, RAC1 and ELMO, mediate cytoskeletal rear-rangements during phagocytosis of apoptotic cells as well as cell motility. Elmo1 associates with DOCK 180 and may influence phagocytosis and effect cell shape changes. Src family kinase-mediated tyrosine phosphorylation of Elmo1 influences signaling through Elmo1/Crk/DOCK 180 pathways. Elmo2 interacts directly with Rho G in a GTP-dependent manner and forms a ternary complex with DOCK 180 to induce activation of Rac 1. The Rho G-Elmo2-DOCK 180 pathway is required for activation of Rac 1 and cell spreading mediated by integrin, as well as for neurite outgrowth induced by nerve growth factor. Elmo3 acts in assocation with DOCK 180 and Crk II and may be required in complex with DOCK 180 to activate Rac/Rho small GTPases.
背景文献
1. Gumienny T L et al. CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. Cell 107:27-41 (2001).
2. Brugnera E et al. Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex. Nat Cell Biol 4:574-582 (2002).
Yes
