产品详情
产品名称eIF4A3 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JG35-33
纯化ProA affinity purified
应用WB,ICC,IF,IHC,FC,IP
种属反应性Hu, Ms, Rt
免疫原描述Recombinant protein within human eIF4A3 aa 1-150.
别名ATP-dependent RNA helicase DDX48 antibody
ATP-dependent RNA helicase eIF4A-3 antibody
DDX48 antibody
DEAD box protein 48 antibody
eIF-4A-III antibody
eIF4A-III antibody
EIF4A3 antibody
eIF4AIII antibody
Eukaryotic initiation factor 4A-III antibody
Eukaryotic initiation factor 4A-like NUK-34 antibody
Eukaryotic translation initiation factor 4A isoform 3 antibody
hNMP 265 antibody
IF4A3_HUMAN antibody
NMP 265 antibody
NMP265 antibody
Nuclear matrix protein 265 antibody
NUK34 antibody
数据库入口号Swiss-Prot#:P38919
计算分子量47 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of eIF4A3 on SiHa cell lysates using anti-eIF4A3 at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-eIF4A3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-eIF4A3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-eIF4A3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-eIF4A3 antibody. Counter stained with hematoxylin.
ICC staining eIF4A3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining eIF4A3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining eIF4A3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of MCF-7 cells with eIF4A3 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
背景
ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation.
背景文献
1. Bertram K. et al. Cryo-EM structure of a human spliceosome activated for step 2 of splicing. Nature 542:318-323(2017)
2. Favaro F.P., et al. A noncoding expansion in EIF4A3 causes Richieri-Costa-Pereira syndrome, a craniofacial disorder associated with limb defects. Am. J. Hum. Genet. 94:120-128(2014)
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