产品详情
产品名称Emi1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JG35-83
纯化ProA affinity purified
应用WB,ICC,IHC
种属反应性Hu, Ms, Rt
免疫原描述Recombinant protein within human Emi1 aa 50-250.
别名Early mitotic inhibitor 1 antibody
Emi 1 antibody
EMI1 antibody
F box only protein 5 antibody
F box protein 5 antibody
F box protein Fbx 5 antibody
F box protein Fbx5 antibody
F-box only protein 5 antibody
FBX5 antibody
FBX5_HUMAN antibody
Fbxo31 antibody
fbxo5 antibody
数据库入口号Swiss-Prot#:Q9UKT4
计算分子量50 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
IHC: 1:50-1:200
ICC: 1:50-1:200
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Emi1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Emi1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-Emi1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Emi1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Emi1 antibody. Counter stained with hematoxylin.
ICC staining Emi1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Emi1 in Siha cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Emi1 (for early mitotic inhibitor) regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry. Emi1 is required to arrest unfertilized eggs at metaphase of meiosis II and may be the long-sought mediator of CSF activity. Human Emi1 is similar to Xenopus laevis Emi1, which inhibits the APC (Cdc20) ubiquitination complex to allow accumulation of cyclin B.
背景文献
1. Hsu J Y et al. E2F-dependent accumulation of hEmi1 regulates S phase entry by inhibiting APC(Cdh1). Nat Cell Biol 4:358-366 (2002).
2. Miller J J et al. Emi1 stably binds and inhibits the anaphase-promoting complex/cyclosome as a pseudosubstrate inhibitor. Genes Dev 20:2410-2420 (2006).
Yes
