NDUFB8 Rabbit mAb - SAB | Signalway Antibody

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NDUFB8 Rabbit mAb#49897

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Recombinant

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产品详情

产品名称NDUFB8 Rabbit mAb

克隆号JG61-36

纯化ProA affinity purified

应用WB,IP,IHC,FC,ICC，IF

种属反应性Hu, Ms, Rt

免疫原描述Recombinant protein within human NDUFB8 aa 30-130.

别名ASHI antibody
CI-ASHI antibody
Complex I ASHI subunit antibody
Complex I-ASHI antibody
mitochondrial antibody
NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa antibody
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 antibody
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial antibody
NADH-ubiquinone oxidoreductase ASHI subunit antibody
NDUB8_HUMAN antibody
NDUFB8 antibody

数据库入口号Swiss-Prot#:O95169

计算分子量17 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情

WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50
IP: 1:10-1:50
FC: 1:50-1:100
IF 1:50-1:200

Western blot analysis of NDUFB8 on different lysates using anti-NDUFB8 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293
Lane 2: A549
Lane 3: Mouse heart
Lane 4: Rat spleen

Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.

Flow cytometric analysis of 293Tcells with NDUFB8 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

背景

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

背景文献

1. Stroud D A et al. Accessory subunits are integral for assembly and function of human mitochondrial complex I. Nature 538:123-126 (2016).
2. Murray J et al. The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification. J Biol Chem 278:13619-13622 (2003).

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应用

WB免疫印迹
IHC免疫组化
IF免疫荧光
ICC免疫细胞化学
FC流式细胞
IP免疫沉淀
E酶联免疫吸附法
DB免疫斑点法
ChIP染色质免疫沉淀
GICA胶体金免疫层析法
NC阴性对照

种属反应性

Hu人
Ms小鼠
Rt大鼠
Dm果蝇
C线虫
Mk猴
Rb兔
B牛
D狗
P猪
Hm仓鼠
ChHm中国仓鼠
Chk鸡

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NDUFB8 Rabbit mAb#49897

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