产品详情
产品名称DAP12 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JG38-70
纯化ProA affinity purified
应用WB,IHC,FC
种属反应性Hu
免疫原描述Synthetic peptide within human DAP12 aa 60-100.
别名DAP 12 antibody
DAP12 antibody
DNAX activation protein 12 antibody
DNAX-activation protein 12 antibody
KAR-associated protein antibody
KARAP antibody
Killer activating receptor associated protein antibody
Killer-activating receptor-associated protein antibody
PLOSL antibody
TYOBP_HUMAN antibody
TYRO protein tyrosine kinase binding protein antibody
TYRO protein tyrosine kinase-binding protein antibody
TYROBP antibody
数据库入口号Swiss-Prot#:O43914
计算分子量12 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of DAP12 on THP-1 cell using anti-DAP12 antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-DAP12 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of K562 cells with DAP12 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
背景
Natural killer (NK) cells are regulated by stimulatory and inhibitory signals from a variety of receptors. Three main receptor families are responsible for NK cells recognition of MHC I molecules, including Ly-49, CD94/NKG2 and KIR (killer-cell inhibitory receptor). DAP12 is a phosphoprotein that is involved in the activation of NK cells. This protein interacts with membrane glycoproteins of the KIR family, resulting in cellular activation. DAP12 also binds to CD94/NKG2C, an activating NK cell receptor belonging to the C-type lectin superfamily. Additional proteins that bind to DAP12 include Ly-49D and Ly-49H, which associate with DAP12 in the plasma membrane. Phosphorylated DAP12 binds to ZAP-70 and Syk, suggesting that the activation pathway may be similar to that of the T and B cell antigen receptors.
背景文献
1. Paloneva J et al. Loss-of-function mutations in TYROBP (DAP12) result in a presenile dementia with bone cysts. Nat Genet 25:357-361 (2000).
2. Kondo T et al. Heterogeneity of presenile dementia with bone cysts (Nasu-Hakola disease): three genetic forms. Neurology 59:1105-1107 (2002).
Yes
