产品详情
产品名称ORP150 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JG39-11
纯化ProA affinity purified
应用WB,IHC,FC
种属反应性Hu, Ms, Rt
免疫原描述Recombinant protein within human ORP150 aa 800-950.
别名150 kDa oxygen regulated protein antibody
150 kDa oxygen-regulated protein antibody
170 kDa glucose regulated protein antibody
170 kDa glucose-regulated protein antibody
DKFZp686N08236 antibody
FLJ94899 antibody
FLJ97572 antibody
glucose regulated protein 170 antibody
Grp 170 antibody
GRP-170 antibody
Grp170 antibody
HSP 12A antibody
HSP12A antibody
Hyou1 antibody
HYOU1_HUMAN antibody
Hypoxia up regulated 1 antibody
hypoxia up-regulated 1 precursor antibody
Hypoxia up-regulated protein 1 antibody
Hypoxia upregulated 1 antibody
Orp 150 antibody
ORP-150 antibody
Orp150 antibody
oxygen regulated protein (150kD) antibody
数据库入口号Swiss-Prot#:Q9Y4L1
计算分子量111 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:5,000-1:10,000
IHC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of ORP150 on mouse prostate tissue lysate using anti-ORP150 antibody at 1/5,000 dilution.
Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ORP150 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-ORP150 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of HepG2 cells with ORP150 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
背景
ORP150, also known as HYOU1 (hypoxia upregulated 1), is a 999 amino acid protein that localizes to the lumen of the endoplasmic reticulum (ER) and is a member of the heat shock protein 70 (HSP 70) family. ORP150 is highly expressed in tissues that have well-developed ERs and a large number of secretory proteins (such as liver and pancreas). It is expressed at lower levels in kidney and brain, and plays an essential role in cytoprotective cellular responses to hypoxia (oxygen deprivation). Specifically, ORP150 participates in protein folding and secretion in the ER and functions to protect cells from hypoxia-induced apoptosis, thereby playing a crucial role in cell survival. ORP150 expression is upregulated in a variety of tumors, such as breast cancer, suggesting an important role in tumorigenesis. The gene encoding ORP150 has two translation initiation sites, resulting in a truncated transcript that lacks an ER signal peptide, but is thought to function as a housekeeping protein in the cytoplasm.
背景文献
1. Ozawa K et al. 150-kDa oxygen-regulated protein (ORP150) suppresses hypoxia-induced apoptotic cell death. J Biol Chem 274:6397-6404 (1999).
2. Zhang H et al. Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry. Nat Biotechnol 21:660-666 (2003).
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