产品详情
产品名称VAMP1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JG40-19
纯化ProA affinity purified
应用WB,IHC,ICC,IF
种属反应性Hu, Ms, Rt
免疫原描述Full length recombinant protein of human VAMP1.
别名DKFZp686H12131 antibody
SYB 1 antibody
SYB1 antibody
Synaptobrevin 1 antibody
Synaptobrevin-1 antibody
Synaptobrevin1 antibody
VAMP 1 antibody
VAMP-1 antibody
Vamp1 antibody
VAMP1_HUMAN antibody
Vesicle associated membrane protein 1 antibody
Vesicle associated membrane protein 1 synaptobrevin 1 antibody
Vesicle-associated membrane protein 1 antibody
数据库入口号Swiss-Prot#:P23763
计算分子量13 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
Western blot analysis of VAMP1 on different tissue lysates using anti-VAMP1 antibody at 1/2,000 dilution.
Positive control:
Lane 1: Mouse cerebellum
Lane 2: Rat brain
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.
ICC staining VAMP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VAMP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VAMP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined.
背景文献
1. Bourassa C V et al. VAMP1 mutation causes dominant hereditary spastic ataxia in Newfoundland families. Am J Hum Genet 91:548-552 (2012).
2. Bechtel S et al. The full-ORF clone resource of the German cDNA consortium. BMC Genomics 8:399-399 (2007).
Yes
