产品详情
产品名称DIAPH1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JE40-59
纯化ProA affinity purified
应用WB,ICC,IF,IHC
种属反应性Hu
免疫原描述Recombinant protein corresponding to N-terminal human DIAPH1.
别名DIAPH1 antibody
deafness, autosomal dominant 1 antibody
DFNA1 antibody
DIA1 antibody
DIAP1 antibody
DIAP1_HUMAN antibody
DIAPH1 antibody
Diaphanous homolog 1 (Drosophila) antibody
diaphanous homolog 1 antibody
Diaphanous related formin 1 antibody
Diaphanous-related formin-1 antibody
DRF1 antibody
FLJ25265 antibody
hDIA1 antibody
LFHL1 antibody
low frequency hearing loss 1 antibody
p140DIA antibody
Protein diaphanous homolog 1 antibody
数据库入口号Swiss-Prot#:O60610
计算分子量141 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:5,000-1:10,000
IHC: 1:50-1:100
ICC: 1:50
Western blot analysis of DIAPH1 on SiHa cell lysate using anti-DIAPH1 antibody at 1/5,000 dilution.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-DIAPH1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-DIAPH1 antibody. Counter stained with hematoxylin.
ICC staining DIAPH1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
背景
Dia 1, also known as DIAPH1 (diaphanous homolog 1) or DRF1, a mammalian homolog of the Drosophila diaphanous gene, belongs to a family of formin homology (FH) proteins which are characterized by having tandemly aligned FH1 (formin homology 1) and FH2 (formin homology 2) domains in their carboxy terminal regions. Dia 1 contains a DAD (diaphanous autoregulatory) domain, which is involved in the elongation of actin filaments, and a GBD/FH3 (Rho GTPase-binding/formin homology 3) domain, which interacts with the DAD domain via autoinhibitory interactions to regulate the activation of Dia 1. Dia 1 is required for the assembly of F-actin structures, and regulates the polymerization and depolymerization of actin filaments. Localizing to the cell membrane, Dia 1 is expressed in a wide range of tissues, including brain, heart, lung and kidney. Defects to the gene encoding Dia 1 have been linked to deafness autosomal dominant type 1 (DFNA1), a disorder characterized by sensorineural hearing loss..
背景文献
1. Zaoui K et al. ErbB2 receptor controls microtubule capture by recruiting ACF7 to the plasma membrane of migrating cells. Proc Natl Acad Sci USA 107:18517-18522 (2010).
2. Bai S W et al. Identification and characterization of a set of conserved and new regulators of cytoskeletal organisation, cell morphology and migration. BMC Biol 9:54-54 (2011).
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