产品详情
产品名称DDB2 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JE16-41
纯化ProA affinity purified
应用WB,IHC,FC
种属反应性Hu
免疫原描述Recombinant protein with C-terminal human DDB2.
别名damage-specific DNA binding protein 2 antibody
Damage-specific DNA-binding protein 2 antibody
DDB p48 subunit antibody
Ddb2 antibody
DDB2_HUMAN antibody
DDBb antibody
DNA damage-binding protein 2 antibody
UV-damaged DNA-binding protein 2 antibody
UV-DDB 2 antibody
Xeroderma pigmentosum group E protei antibody
数据库入口号Swiss-Prot#:Q92466
计算分子量48 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
IHC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of DDB2 on Daudi cell using anti-DDB2 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-DDB2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-DDB2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-DDB2 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of A549 cells with DDB2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
背景
Damaged DNA binding protein (DDB) is a heterodimer composed of two subunits, p127 and p48, which are designated DDB1 and DDB2, respectively. The DDB heterodimer is involved in repairing DNA damaged by ultraviolet light. Specifically, DDB, also designated UV-damaged DNA binding protein (UV-DDB), xeroderma pigmentosum group E binding factor (XPE-BF) and hepatitis B virus X-associated protein 1 (XAP-1), binds to damaged cyclobutane pyrimidine dimers (CPDs). Mutations in the DDB2 gene are implicated as causes of xeroderma pigmentosum group E, an autosomal recessive disease in which patients are defective in nucleotide excision DNA repair. XPE is characterized by hypersensitivity of the skin to sunlight with a high frequency of skin cancer as well as neurologic abnormalities. The hepatitis B virus (HBV) X protein interacts with DDB1, which may mediate HBx transactivation.
背景文献
1. Inoki T et al. Human DDB2 splicing variants are dominant negative inhibitors of UV-damaged DNA repair. Biochem Biophys Res Commun 314:1036-1043 (2004).
2. Hwang B J et al. Expression of the p48 xeroderma pigmentosum gene is p53-dependent and is involved in global genomic repair. Proc Natl Acad Sci USA 96:424-428 (1999).
Yes
