Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ABP1 in samples. An antibody specific for ABP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:On the basis of its primary structure, the amiloride-binding protein (EC 1.4.3.6) is 713 amino acids long, with a 19-amino acid signal peptide. Expressed in cultured cells, the mRNA yields a glycoprotein that binds amiloride and amiloride analogs with affinities similar to the amiloride receptor associated with the apical Na+ channel in pig kidney membranes and is immunoprecipitated with monoclonal antibodies raised against pig kidney amiloride-binding protein. Barbry et al. (1990) pointed out that amiloride-sensitive Na+ channels are also present in airway epithelia, where they play an important role in fluid secretion. Amiloride inhibits the excessive absorption of Na+ and liquid that takes place in airway epithelia of patients with cystic fibrosis, and amiloride aerosol therapy has been tried for the treatment of lung disease in CF.