Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PEDF in samples. An antibody specific for PEDF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPEDF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PEDF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PEDF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The PEDF gene is a member of the serpin gene family. Serpins are a group of serine protease inhibitors, some of which have also been reported to exhibit neurotrophic activity.By the analysis of 3 independent somatic cell hybrid panels, Tombran-Tink et al. (1994) assigned the PEDF gene to chromosome 17. Fluorescence in situ hybridization showed localization at the terminal portion of 17p. PCR analysis of somatic cell hybrids containing specific regions of 17 were subsequently used to sublocalize PEDF to 17pter-p13.1. Greenberg et al. (1997) used linkage analysis to narrow the localization of the PEDF gene to 17p13.3, the same region as that carrying the autosomal dominant retinitis pigmentosa locus (RP13) identified in a South African family.