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位置: 首页 > 磷酸化抗体 > DLC1 (Phospho-Ser986) Antibody

DLC1 (Phospho-Ser986) Antibody#11592

DLC1 (Phospho-Ser986) Antibody
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产品总价:
产品详情

产品名称DLC1 (Phospho-Ser986) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of DLC1 only when phosphorylated at serine 986.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 986 (R-D-S(p)-G-V) derived from Human DLC1.

基因/蛋白名称DLC1

修饰Phospho

别名ARHGAP7, KIAA1723, STARD12

数据库入口号Swiss-Prot: Q96QB1
NCBI Protein: NP_872584.2.

UniprotQ96QB1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 171kd 114kd 123kd

Western blotting: 1:500~1:1000

Western blot analysis of extracts from DU145 cells using DLC1 (Phospho-Ser986) Antibody #11592.
背景

Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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