Bcl-2-like protein 1 Monoclonal Antibody - SAB

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Bcl-2-like protein 1 Monoclonal Antibody#42016

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Bcl-2-like protein 1 Monoclonal Antibody

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产品名称Bcl-2-like protein 1 Monoclonal Antibody

来源种属Mouse

克隆性Monoclonal

纯化protein G purifed

应用WB IHC

种属反应性Hu

特异性specific for Human Bcl-2-like protein 1 denatured and native forms

免疫原类型protein

免疫原描述Recombinant Human Bcl-2-like protein 1 protein

基因/蛋白名称Bcl-2-like protein 1

别名Bcl2-L-1, Apoptosis regulator Bcl-X, BCL2L1, BCL2L, BCLX

数据库入口号Swiss-Prot#: Q07817

UniprotQ07817

计算分子量26kd

浓度1.0mg/mL

配方Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

保存Store at -20˚C

应用详情

Western blotting: 1:500 - 1:1000
Immunohistochemistry: 1:20 - 1:200

All Lanes:Mouse anti BCL2L1 Monoclonal antibody at 1ug/ml Lane 1:HepG2 whole cell lysate Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution Predicted band size:26KD Observed band size:26KD

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue using #42016 at dilution of 1:200.

Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue using #42016 at dilution of 1:200.

背景

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.

背景文献

"bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death." Boise L.H., Gonzalez-Garcia M., Postema C.E., Ding L., Lindsten T., Turka L.A., Mao X., Nunez G., Thompson C.B. Cell 74:597-608(1993) [PubMed] [Europe PMC] [Abstract]

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操作手册

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应用

WB免疫印迹
IHC免疫组化
IF免疫荧光
ICC免疫细胞化学
FC流式细胞
IP免疫沉淀
E酶联免疫吸附法
DB免疫斑点法
ChIP染色质免疫沉淀
GICA胶体金免疫层析法
NC阴性对照

种属反应性

Hu人
Ms小鼠
Rt大鼠
Dm果蝇
C线虫
Mk猴
Rb兔
B牛
D狗
P猪
Hm仓鼠
ChHm中国仓鼠
Chk鸡

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Bcl-2-like protein 1 Monoclonal Antibody#42016

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