Osteopontin Monoclonal Antibody - SAB

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Osteopontin Monoclonal Antibody#42036

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产品名称Osteopontin Monoclonal Antibody

来源种属Mouse

克隆性Monoclonal

纯化protein G purifed

应用WB IHC

种属反应性Hu

特异性specific for human Osteopontin denatured and native forms

免疫原类型protein

免疫原描述Recombinant Human Osteopontin

基因/蛋白名称Osteopontin

别名Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, BNSP, OPN, PSEC0156

数据库入口号Swiss-Prot#: P10451

UniprotP10451

计算分子量34.5kd

浓度1.0mg/mL

配方Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

保存Store at -20˚C

应用详情

Western blotting: 1:500 - 1:1000
Immunohistochemistry: 1:20 - 1:200

All Lanes Mouse anti human OPN monoclonal antibody at at 2 ug/ml Lane 1 mouse kidney tissue lysate Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution Predicted band size : 34.5 kDa Observed band size: 35kDa Additional bands at : 50 kDa. We are unsure as to the identity of this extra band.

Immunohistochemical analysis of paraffin-embedded human colon cancer using #42036 at dilution of 1:200.

Immunohistochemical analysis of paraffin-embedded human Kidney tissue using #42036 at dilution of 1:200.

背景

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity

背景文献

[1] Kiefer M.C., Bauer D.M., Barr P.J. The cDNA and derived amino acid sequence for human osteopontin. Nucleic Acids Res. 17:3306-3306(1989) [2] Young M.F., Kerr J.M., Termine J.D., Wewer U.M., Wang M.G., McBride O.W., Fisher L.W. cDNA cloning, mRNA dist

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应用

WB免疫印迹
IHC免疫组化
IF免疫荧光
ICC免疫细胞化学
FC流式细胞
IP免疫沉淀
E酶联免疫吸附法
DB免疫斑点法
ChIP染色质免疫沉淀
GICA胶体金免疫层析法
NC阴性对照

种属反应性

Hu人
Ms小鼠
Rt大鼠
Dm果蝇
C线虫
Mk猴
Rb兔
B牛
D狗
P猪
Hm仓鼠
ChHm中国仓鼠
Chk鸡

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