产品详情
产品名称Myoglobin Monoclonal Antibody
来源种属Mouse
克隆性Monoclonal
纯化protein G purifed
应用WB
种属反应性Hu
特异性specific for Human Myoglobin denatured and native forms
免疫原类型protein
免疫原描述Recombinant Human Myoglobin protein
基因/蛋白名称Myoglobin
别名myoglobin;MB;MGC13548;PVALB
数据库入口号Swiss-Prot#: P02144
UniprotP02144
计算分子量17kd
浓度1.0mg/mL
配方Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
保存Store at -20˚C
应用详情
Western blotting: 1:500 - 1:1000
Immunohistochemistry: 1:20 - 1:200
All lanes :Mouse anti-Human Myoglobin monoclonal antibody at 0.3ug/ml
Lane 1:mouse heart muscle lysate
Lane 2:Recombinant Myoglobin at 10ug
Secondary HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilution
Predicted band size : 17kd
Observed band size : 20kd
Additional bands at:120KD(We are unsure as to the identity of this extra band.)
All lanes :Mouse anti-Human Myoglobin monoclonal antibody at 1ug/ml
Lane 1:Myoglobin transfected 293 cell lysate
Secondary
HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilution
Predicted band size : 17kd
Observed band size : 20kd
Additional bands at:23/25/40KD(We are unsure as to the identity of this extra band.)
Immunohistochemical analysis of paraffin-embedded human heart tissue using #42039 at dilution of 1:100.
背景
Myoglobin is a small heme containing protein (153 amino acid residues, molecular weight (w/o heme) 17053 Da and theoretical pI=7.29) responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1 to 3 hours after onset of the symptoms, reaching peak level within 8 to 12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.
背景文献
[1] Karabay O, Tuna N, Ogutlu A, Gozdas HT.High ferritin and myoglobin level in legionella pneumonia: a case report and review of literature. Indian J Pathol Microbiol. 2011 Apr-Jun;54(2):381-3.[2] Liu M, Hou M, Liu LP, Zhang CX, Zhao CY, Ma CJ, Zhong S
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