Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LAMA5 in samples. An antibody specific for LAMA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAMA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Components of the extracellular matrix exert myriad effects on tissues throughout the body. In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. Laminin subunit alpha-5 belongs to the alpha subfamily of laminin chains and is a major component of basement membranes.