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位置: 首页 > ELISA试剂盒 > Rat Kidney injury molecule 1 (Kim-1) ELISA Kit

Rat Kidney injury molecule 1 (Kim-1) ELISA Kit#EK10388

是否有货: 1-2周
产品总价:
产品详情

产品名称Rat Kidney injury molecule 1 (Kim-1) ELISA Kit

简述ELISA Kit

应用ELISA

种属反应性Rat (Rattus norvegicus)

别名HAVCR; HAVCR-1; KIM-1; KIM1; TIM-1; TIM1; TIMD1; T cell immunoglobin domain and mucin domain protein 1|kidney injury molecule 1

数据库入口号O54947

保存The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).

应用详情
Detect Range:0.31-20 ng/mL
Sensitivity:0.156 ng/mL
Sample Type:Serum, Plasma, Other biological fluids
Sample Volume: 1-200 μL
Assay Time:1-4.5h
Detection wavelength:450 nm
产品描述
Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate HAVCR1 in samples. An antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHAVCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAVCR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAVCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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