免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 磷酸化抗体 > Rb(Phospho-Ser807) Antibody

Rb(Phospho-Ser807) Antibody#11131

Rb(Phospho-Ser807) Antibody
Rb(Phospho-Ser807) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Rb(Phospho-Ser807) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Rb only when phosphorylated at serine 807.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 807 (Y-I-S(p)-P-L) derived from Human Rb.

基因/蛋白名称Rb

修饰Phospho

别名P105-RB; PP105; PP110; RB-1; RB1

数据库入口号Swiss-Prot: P06400
NCBI Protein: NP_000312.2

UniprotP06400

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 3T3 cells untreated or treated with UV using Rb(Phospho-Ser807) Antibody #11131.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Phospho-Ser807) Antibody #11131(left) or the same antibody preincubated with blocking peptide(right).
背景
Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Roesch A, et al. (2005) Mod Pathol. 18(4): 565-572.
Chadee DN, et al. (2004) Nat Cell Biol. 6(8): 770-776.
Knudsen ES, et al. (1997) Mol Cell Biol. 17(10): 5771-5783.
Knudsen ES, et al. (1996) J Biol Chem. 271(14): 8313-8320.

如果您使用该产品11131发表了文章,请通知我们,让我们可以引用您的文献。

et al,The reversal effect of Ginsenoside Rh2 on drug resistance in human colorectal carcinoma cells and its mechanism. In Hum Cell.On 2018 Jul by Liu GW, Liu YH et al..PMID:29582366, , (2018),
PMID: 29582366
et al,Vinpocetine Inhibits Breast Cancer Cells Growth in Vitro and in Vivo.In Apoptosis on 2012 Oct by Er-Wen Huang, Sheng-Jiang Xue, et al..PMID:22729609, , (2012),
PMID: 22729609
et al,Luteolin induced G2 phase cell cycle arrest and apoptosis on non-small cell lung cancer cells.In Toxicol In Vitro on 2011 Oct by Cai X, Ye T,et al..PMID:21601631, , (2011),
PMID: 21601631
Qin Liu, Hanjiang Fu, Fang Sun et al,miR-16 family induces cell cycle arrest by regulating multiple cell cycle genes., Nucleic Acids Res., 36: 5391-5404 (2008),
PMID: 18701644
et al,miR-16 family induces cell cycle arrest by regulating multiple cell cycle genes. In Nucleic Acids Res on 2008 Sep by Qin Liu, Hanjiang Fu, et al..PMID: 18701644, , (2008),
PMID: 18701644
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com