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位置: 首页 > ELISA试剂盒 > Mouse 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1) ELISA Kit

Mouse 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1) ELISA Kit#EK10940

是否有货: 1-2周
产品总价:
产品详情

产品名称Mouse 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1) ELISA Kit

简述ELISA Kit

应用ELISA

种属反应性Mouse (Mus musculus)

别名CBAS3; CP7B; SPG5A; cytochrome P450; subfamily VIIB (oxysterol 7 alpha-hydroxylase); polypeptide 1|oxysterol 7alpha-hydroxylase

数据库入口号Q60991

保存The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).

应用详情
Detect Range:Request Information
Sensitivity:Request Information
Sample Type:Serum, Plasma, Other biological fluids
Sample Volume: 1-200 μL
Assay Time:1-4.5h
Detection wavelength:450 nm
产品描述
Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate CYP7B1 in samples. An antibody specific for CYP7B1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP7B1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP7B1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP7B1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

如果您使用该产品EK10940发表了文章,请通知我们,让我们可以引用您的文献。

et al,Hepatic miR-378 modulates serum cholesterol levels by regulating hepatic bile acid synthesis. In Theranostics on 2021 Feb 25 by Chao Sun, Wei Liu, et al..PMID:33754066, , (2021),
PMID: 33754066
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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