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位置: 首页 > ELISA试剂盒 > Bovine Progesterone (PROG) ELISA Kit

Bovine Progesterone (PROG) ELISA Kit#EK11168

是否有货: 1-2周
产品总价:
产品详情

产品名称Bovine Progesterone (PROG) ELISA Kit

简述ELISA Kit

应用ELISA

种属反应性Bovine (Bos taurus; Cattle)

保存The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).

应用详情

Detect Range:2 ng/mL - 140 ng/mL.
Sensitivity:0.5ng/mL
Sample Type:Serum, Plasma, Other biological fluids
Sample Volume: 50 μL
Assay Time:1-4.5h
Detection wavelength:450 nm

产品描述

Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PROG in samples. An antibody specific for PROG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROG bound in the initial step. The color development is stopped and the intensity of the color is measured.

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5

注释

应用

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  • DB免疫斑点法
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  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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025-58868422

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